Catecholamines and Metabolites (1) (ODP-50 6D)

Catecholamines and metabolites were analyzed using Asahipak ODP-50 6D.

Sample:

Catecholamines

NE: Norepinephrine

E: Epinephrine

DOPA: 3,4-Dihydroxyphenylalanine

DA: Dopamine

5HT: 5-Hydroxytryptamine

DOMA: 3,4-Dihydroxymandelic acid

MHPG: 3-Methoxy-4-hydroxyphenylglycol

VMA: Vanillylmandelic acid

DOPAC: 3,4-Dihydroxyphenylacetic acid

HVA: Homovanilic acid

5HIAA: 5-Hydroxyindole-3-acetic acid

Column       : Shodex Asahipak ODP-50 6D (6.0mmI.D. x 150mm)
Eluent       : (A); 0.1M H3PO4(pH2.0)
               (B); 0.1M H3PO4(pH2.0)/CH3CN=70/30
               Linear gradient:
               0min to 10min, (A)
               afetr 10min, 2.5%/min gradient of (B)
Flow rate    : 0.8mL/min
Detector     : UV(280nm)