Cation Analysis in the Presence of Albumin (YS-50)

Ion chromatography (cation) column, IC YS-50 was used to separate mono- and divalent cations in the presence of albumin. No considerable influence from the presence of albumin was observed. Part of protein (albumin) may be adsorbed on the column, thus column cleaning using10mM Na₂HPO₄(pH9-9.5) is recommended after the analysis.

Sample: 10μL Cation mixture in 7% Albumin

1. Li⁺
2. Na⁺
3. NH₄⁺
4. K⁺
5. Mg²⁺
6. Ca²⁺

Column       : Shodex IC YS-50 (4.6mmI.D. x 125mm)
Eluent       : 4mM Methanesulfonic acid aq.
Flow rate    : 1.0mL/min
Detector     : Non-suppressed conductivity
Column temp. : 40°C