Ion chromatography (cation) column, IC YS-50 was used to separate mono- and divalent cations in the presence of albumin. No considerable influence from the presence of albumin was observed. Part of protein (albumin) may be adsorbed on the column, thus column cleaning using10mM Na2HPO4(pH9-9.5) is recommended after the analysis.
Sample: 10μL Cation mixture in 7% Albumin
1. Li+
2. Na+
3. NH4+
4. K+
5. Mg2+
6. Ca2+
Column : Shodex IC YS-50 (4.6mmI.D. x 125mm) Eluent : 4mM Methanesulfonic acid aq. Flow rate : 1.0mL/min Detector : Non-suppressed conductivity Column temp. : 40°C