Chlorpheniramine and Metabolic Intermediates

Chlorpheniramine and methaboric intermediates were analyzed using ORpak CDBS-453 (a column for chiral separation).

Sample :
1. Chlorpheniramine
2. Monodesmethyl-chlorpheniramine
3. Didesmethyl-chlorpheniramine

 Column : Shodex ORpak CDBS-453 (4.6mmID*150mm)
 Eluent : 10% CH3CN 90% (1.0% CH3COOH + 0.2M NaCl) in H2O
 Flow rate : 0.5mL/min
 Detector : UV(254nm)
 Column temp. : 30°C

Courtesy of Dr. Sakurai, Tohoku Cllage of Pharmacy