Saccharides in chocolate cake were analyzed using SUGAR SZ5532 (a column for saccharides analysis).
Sample pretreatment
1) Take 5g of chocolate cake in a vial for centrifuge, add 40 mL of petroleum ether and stir it for 15 minutes. Then use a centrifuge for 10 minutes at 2,000 rpm and take off the supernatant.
2) Again, add 40 mL of petroleum ether and stir it for 15 minutes and take off the remaining petroleum ether completely in a water bath of 40 °C.
3) Add 30 mL of pure water, apply ultrasonic vibration for 30 minutes and make 50 mL of solution with pure water.
4) Filtrate with No.5B paper filter and ultrafiltrate at Mw 10,000.
5) Take a part of the filtrated sample and add the same quantity of acetonitrile.
6) Filtrate with a 0.45-micron membrane filter.
Sample: Chocolate cake, 10micro-L
1. Fructose
2. Glucose
3. Sucrose
4. Maltose
5. Lactose
Column : Shodex SUGAR SZ5532 (6.0mmID*150mm) Eluent : CH3CN/H2O=75/25 Flow rate : 0.6mL/min Detector : Shodex RI Column temp. : 60°C
