Chondroitin sulfate is an acidic mucopolysaccharide which has a chain alternating disaccharide units composed of N-acetyl-D-galactosamine and D-glucuronic acid; it is found in animal tissues. There are seven kinds of chondroitin sulfate (A, B, C, D, E, H and K) which differ in positions and the number of sulfate groups. Chondroitin sulfate B, which is known as dermatan sulfate, contains the L-iduronic acids in place of D-glucuronic acids as uronic acids. In this application, three kinds of chondroitin sulfate were analyzed using OHpak SB-806M HQ, a column for aqueous SEC column.
Sample: 0.1% each, 50μL.
Columns : Shodex OHpak SB-806M HQ (8.0mmI.D. x 300mm) x 2 Eluent : 0.1M NaNO3 aq. Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 40°C