The ligand of AFpak AST-894 (an affinity column) is soybean trypsin inhibitor (STI). The column was used to adsorb and elute chymotrypsin which is a typical digestive enzyme.
Sample : Chymotrypsin
Column : Shodex AFpak AST-894 (8.0mmID*50mm)
Eluent : (A); 0.1M Sodium acetate buffer(pH7.7) + 0.5M NaCl
(B); 0.1M Sodium acetate buffer(pH2.8) + 0.5M NaCl
Linear gradient: 0min to 20min, (A), 20min to 40min, (A) to (B), after 40min, (B)
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : Room temp.