Collagen and BSA

Collagen and BSA were analyzed using Asahipak ES-502N 7C (a column for weak anion exchange chromatography).

Sample :
1. Collagen
Pepsin-digested product of collagen
soluble in acid extracted from pig skin
2. BSA

 Column : Shodex Asahipak ES-502N 7C (7.6mmID*100mm)
 Eluent : 20mM Monoethanolamine buffer(pH9.5) + 500mM NaCl 
 Flow rate : 1.0mL/min
 Detector : UV(280nm)
 Column temp. : 30°C
 

Courtesy of Dr. Shirai, Tokyo University of Agriculture and Technology