When a column is deteriorated by the foreign substances remaining in the column
or adsorbed by the packing material, the substances might be washed out by the
procedure described below. However, sometimes the procedure is not sufficient to
regenerate the column and, in such case, it is necessary to replace the column
with a new one. And, even if the column can be regenerated, the column performance
may be not so good as before. After cleaning the column, be sure to replace the
in-column solvent to the normal solvent. The use of a guard column at the up-stream
of the main column is recommended to prevent the main column from the deterioration
by the foreign substances. When a guard column is used, first, the guard column
will be deteriorated by the foreign substances and it is possible to prevent the
main column from the deterioration by replacing the guard column regularly.
When cleaning a guard column, be sure to disconnect the main column before
the cleaning. If a guard column and a main column are
connected in series and the cleaning procedure
is performed, foreign substances in the guard
column may move to the main column and, as
the consequence, the main column may be deteriorated.
| Category | Column name | Coulmn cleaning procedure | |
|---|---|---|---|
| GPC Clean-up | CLNpak | EV | Reverse the column on the HPLC system, flow the eluent at 80% of ordinary flow rate. This is effective for the foreign substances remaining in the column, however not effective for the substances adsorbed to the packing material. |
| CLNpak | PAE | ||
| Reversed Phase,
Hydrophilic Interaction |
Asahipak | ODP, C8P, C4P | Flow 50mL of dioxane, CH3CN or methanol. |
| RSpak | RP18, DS | ||
| RSpak | DE, GOLF
CARB |
||
| RSpak | DM-614 | Flow 50mL of methanol, CH3CN or H2O. | |
| RSpak | NN | Inject 20 to 50 µL of 2N nitric acid aqueous solution two or three times. | |
| Asahipak | NH2P-50 4E | (Standard cleaning) Reverse the direction and flow rate is 0.5mL/min. 1) H2O 10min, 2) 0.1M NaOH aq. 120min, 3) H2O 20min, 4) Eluent 60min (Cleaning metal ions) Reverse the direction and flow rate is 0.5mL/min. 1) H2O 10min, 2) 0.1M HClO4 aq. 60min, 3) H2O 10min, 4) 0.1M NaOH aq. 120min, 5) H2O 20min, 6) Eluent 60min | |
| Reversed Phase,
Hydrophilic Interaction |
ODSpak | F | Flow 50mL of dioxane, THF, CH3CN or methanol. |
| Silica | C18M, C18P | ||
| Silicapak | E-411 | Flow 50mL each of n-hexane, dichloroehtane, methanol, dichloroethane and n-hexane. | |
| SIlica | 5SIL | ||
| Silica | 5C8, 5C4, 5CN | Flow 50mL of dioxane, CH3CN or methanol. | |
| Silica | 5NH | There is no appropriate washing method. | |
| Silica | 5NPE | ||
| Silica | 5PYE | ||
| Chiral Separation | ORpak | CDA, CDB, CDC | There is no appropreate cleaning method. |
| ORpak | CDBS, CRX | There is no appropreate cleaning method. | |
| Ion Exchange | AXpak | WA-624 | Inject 1 to 2 mL of 0.1M NaOH aqueous solution or 30% acetic acid aqueous solution. Connect the column in the reverse way and flow the eluent at 0.5mL/min. |
| CXpak | P-421S | ||
| IEC | QA, DEAE
SP, CM |
||
| IEC | DEAE3N-4T
SP-420N |
Inject 500 µL of 0.1M NaOH aqueous solution, 50% formic acid aqueous solution, 20% CH3CN aqueous solution or 20% dimethyl sulfoxide aqueous solution several times. Flow the eluent at 0.3 to 0.5mL/min. | |
| Asahipak | ES-502N | Flow aqueous solution of high salt concentartion or 10mM NaOH aqueous solution for ionic adsorption and 50mM buffer solution containing 50% CH3CN or methanol for hydrophobic adsorption. | |
| Asahipak | ES-502C | Flow aqueous solution of high salt concentartion or 0.1N acetic acid aqueous solution for ionic adsorption and 50mM buffer solution containing 50% CH3CN aqueous solution or methanol for hydrophobic adsorption. | |
| Hydrophobic | HIC | PH-814 | Reverse the column on the HPLC system, flow the eluent at 0.5mL/min and inject 1 to 2 mL of 0.1M NaOH or 30% acetic acid aqueous solution several times. |
| Affinity | AFpak | 31 kinds | Reverse the column on the HPLC system, flow an appropriate eluent at 0.5mL/min. |
| Ligand Exchange | SUGAR | SC1011, 1821 SC1211 | ( for SC1011, 1821)
Flow 50mL of 0.1M Ca(NO3)2 aqueous solution at 50°C, 0.5mL/min. |
| SUGAR | SP0810 | Flow of H2O at 70 – 95 °C, 0.5mL/min. Then inject of 50µL of 0.2M Pb(NO3)2 aqueous 3 or 4 times. | |
| SUGAR | KS-800 | Flow 10mM NaOH aqueous solution at 0.5mL/min. After flowing the solvent, replace the in-column solvent completely with H2O. (Sometimes, injection of 40µL of 0.1M NaOH aqueous solution is will be sufficient.) | |
| RSpak | DC-613 | Flow 50mL of Methanol, Ethanol, CH3CN, or 5mM NaOH aqueous solution. Or, inject 20 to 50 µL of 0.5M NaOH aqueous solution. | |
| SUGAR | SZ5532 | After replacing in-column solvent completely with H2O, flow 30mL of 0.1M Zn(NO3)2 aqueous solution at 50°C, 0.5mL/min. Then, replace the in-column solvent completely with H2O and gradually increase the organic solvent concentration to the concentration to be used. | |
| USPpak | MN-431 | Flow 50mL of 0.1M Ca(NO3)2 aqueous solution at 50°C, 0.5mL/min. | |
| Ion Exclusion | SUGAR | SH1011,1821 | Flow 50mL of 25mM H2SO4 aqueous solution at 50°C, 0.5mL/min. |
| RSpak | KC-811 | Flow 50mL of 25mM H2SO4 aqueous solution at 50°C, 0.5mL/min. | |
| Ion Chromatography | IC | I-524A | –Pollution by accumulation of metals–
30minutes: 0.01M tartaric acid (pH3 to 4, adjust with 0.1M NaOH), 1.0mL/min |
| IC | NI-424 | Flow the mixture of eluent/methanol=90/10 at 0.6mL/min for two hours. | |
| IC | Y-521 | Inject 100µL of 1M HNO3 aqueous solution several times. | |
| IC | YK-421 | Flow 50mL of 50mM tartaric acid aqueous solution or 50mM tartaric acid aqueous solution/CH3CN=50/50 at 0.3mL/min. | |
| IC | YS-50 | Flow 50mL of 4mM Methanesulfonic acid aqueous solution /CH3CN=70/30 at 1.0mL/min. | |
| IC | SI-90 | –Pollution by low valency hydrophilic ions–
1. 15 minutes: deionized water |
|
| IC | SI-50 | –Pollution by low valency hydrophilic ions–
(flow rate 0.3mL/min) |
|
| IC | T-521 | Flow 0.1M NaOH aqueous solution at 0.5mL/min for one hour and 0.1M Nitric acid aqueous solution at 0.5mL/min for one hour. | |
| IC | R-621 | ||
| Aqueous SEC (GFC) | OHpak | SB-800 HQ | Reverse the column on the HPLC system, flow the eluent at 50% of ordinary flow rate. This is effective for the foreign substances remaining in the column, however not effective for the substances adsorbed to the packing material. |
| SB400 | |||
| PROTEIN | KW-800 | ||
| KW400 | |||
| Maltimode | Asahipak | GS-HQ | |
| Aqueous / Organic SEC | Asahipak | GF-HQ | |
| Organic SEC (GPC) | GPC | KF-800 KF-400HQ KF-600 K-800 KD-800 LF HFIP-800 HFIP-600 HT-800 UT-800 AT-806S | |
