Column Test Conditions for Ion Exchange Chromatography
We are often asked “What standards should I run to assess the condition of my column?”. Well, here they are for all of our columns. We realize that chromatographers may have columns for years along with several changes of ownership and/or locations. Often, the original COA is lost. We can replace your COA on request. However, you can run the standard samples and asses the column quality even if you no longer have the original COA. Refer to the conditions below. Don’t hesitate to contact us if you need further assistance. Sometimes the column can be regenerated to produce “good as new” quality chromatograms.
| Column type | Test conditions (1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector |
|---|---|
| SP-825 | (1)0.5% Acetone (2)20μL (3)50mM Na2SO4 aq. (4)1.0mL/min (5)Ambient (6)UV(280nm) |
| SP-420N | (1)0.2% Glycyl-L-tyrosine (2)1μL (3)20mM Acetate buffer + 0.5M Na2SO4(pH5.0) (4)1.0mL/min (5)Ambient (6)UV(280nm) |
| SP-2B | (1)0.2% Glycyl-L-tyrosine (2)1μL (3)20mM Acetate buffer + 0.5M Na2SO4 (pH5.0) (4)0.2mL/min (5)Ambient (6)UV(280nm) |
| CM-825 | (1)0.5% Acetone (2)20μL (3)50mM Na2SO4 aq. (4)1.0mL/min (5)Ambient (6)UV(280nm) |
| ES-502C 7C | (1)0.025% Cytosine (2)15μL (3)100mM Sodium phosphate buffer(pH4.4) (4)1.0mL/min (5)30°C (6)UV(250nm) |
| P-421S | (1)1% Ethylene glycol (2)4μL (3)H2O (4)0.5mL/min (5)Ambient (6)RI |
| QA-825 | (1)0.5% Acetone (2)20μL (3)50mM Na2SO4 aq. (4)1.0mL/min (5)Ambient (6)UV(280nm) |
| DEAE-825 | (1)0.5% Acetone (2)20μL (3)50mM Na2SO4 aq. (4)1.0mL/min (5)Ambient (6)UV(280nm) |
| DEAE3N-4T | (1)200mg/L NaNO3 (2)5μL (3)30mM phosphate buffer(pH6.0) + 0.2M NaCl (4)1.0mL/min (5)Ambient (6)UV(210nm) |
| DEAE-2B | (1)0.1% NaNO3 (2)1μL (3)50mM Na2SO4 aq. (4)0.05mL/min (5)Ambient (6)RI |
| ES-502N 7C | (1)0.025% Xanthine (2)10μL (3)50mM 1,3-Diaminopropane + 50mM NaCl(pH10.0) (4)1.0mL/min (5)30°C (6)UV(250nm) |
| WA-624 | (1)0.03% UMP (2)10μL (3)0.1M Sodium phosphate buffer(pH3.0)/CH3CN=80/20 (4)1.0mL/min (5)Ambient (6)UV(260nm) |
