To check the deterioration of column performance, measure the column plate number and compare it with the number written in the inspection data sheet which is attached to the column. The test conditions for calculation of the plate number is determined for each column type and shown in the table below.
When you first use a column, we recommend you to measure its plate number before use. Plate number (N) can be calculated by the formula in the following figure.
| Column type | Test conditions (1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector |
|---|---|
| ODP-50 6D ODP-50 6E ODP-50 4B ODP-50 4D ODP-50 4E ODP-50 2D |
(1)0.75 μL/mL n-Hexyl benzoate (ODP-50 6D, ODP-50 6E, ODP-50 4B, ODP-50 4D, ODP-50 4E) 0.08 % n-Hexyl Benzoate (ODP-50 2D) (2)10 μL (ODP-50 6D), 17 μL (ODP-50 6E), 3 μL (ODP-50 4B), 6 μL (ODP-50 4D, ODP-50 4E.), 7 μL (ODP-50 2D), (3)CH3CN/H2O=65/35 (4)1.0 mL/min (ODP-50 6D, ODP-50 6E), 0.6 mL/min (ODP-50 4B, ODP-50 4D, ODP-50 4E), 0.2 mL/min (ODP-50 2D) (5)30 °C (ODP-50 6D, ODP-50 6E, ODP-50 4B, ODP-50 4D, ODP-50 4E), Ambient (ODP-50 2D) (6)UV (254 nm) |
| C4P-50 4D | (1)0.75 μL/mL n-Hexyl benzoate (2)6 μL (3)CH3CN/H2O=65/35 (4)0.6 mL/min (5) 30 °C (6)UV (254 nm) |
| ODP2 HP-4B ODP2 HP-4D ODP2 HP-4E ODP2 HP-2B ODP2 HP-2D |
(1)1,000 mg/L Toluene (2)5 μL (ODP2 HP-4B, ODP2 HP-4D, ODP2 HP-4E), 2 μL (ODP2 HP-2B), 1 μL (ODP2 HP-2D) (3)H2O/CH3CN=55/45 (4)0.5 mL/min (ODP2 HP-4B, ODP2 HP-4D, ODP2 HP-4E), 0.1 mL/min (ODP2 HP-2B, ODP2 HP-2D) (5)40 °C (6)UV (254 nm) |
| DS-613 | (1)0.1 % Benzene (2)20 μL (3) CH3CN/THF/H2O=40/30/30 (4)0.8 mL/min (5)Ambient (6)UV (254 nm) |
| DS-413 | (1)1 % 3-Pentanone (2)5 μL (3) CH3CN/THF/H2O=30/30/40 (4)0.6 mL/min (5)Ambient (6)UV (262 nm) |
| DE-613 | (1)0.2 % Ethylene glycol (2)20 μL (3) H2O (4)0.8 mL/min (5)Ambient (6)RI |
| DE-413 | (1)0.6 % Di-n-butyl ketone (2)10 μL (DE-413), 20 μL (DE-413L) (3) CH3CN/H2O=50/50 (4)1.0 mL/min (5)40 °C (6)UV (262 nm) |
| DE-213 | (1)0.6 % Di-n-butyl ketone (2)5 μL (3) CH3CN/H2O=50/50 (4)0.2 mL/min (5)40 °C(6)UV (262 nm) |
| DM-614 | (1)2 % Oxalic acid (2)5 μL (3)5 mM H3PO4 aq. (4)0.8 mL/min (5)Ambient (6)RI |
| NN-814 | (1)0.1 % Uracil (2)10 μL (3)0.1 M NaH2PO4 (pH3.0) (4)1.0 mL/min (5)35 °C (6)UV (260 nm) |
| JJ-50 2D | (1)1.0 % Acetone (2)0.5 μL (3)CH3CN/H2O=60/40 (4)0.15 mL/min (5)Ambient (6)UV (280 nm) |
| VG-50 4D VG-50 4E |
(1)5 mg/mL Sucrose (2)5 μL (3)CH3CN/H2O=80/20 (4)0.6 mL/min (VG-50 4D), 1.0 mL/min (VG-50 4E) (5)40 °C (6)RI |
| VG-50 2D | (1)2 mg/mL Sucrose (2)1 μL (3)CH3CN/H2O=85/15 (4)0.2 mL/min (5)40 °C (6)RI |
| VT-50 2D | (1)250 µg/mL Allantoin (2)2 μL (3)CH3CN/25 mM HCOONH4=85/15 (4)0.2 mL/min (5)40 °C (6)UV (210 nm) |
| VC-50 2D | (1)50 µg/mL Uracil (2)2 μL (3)CH3CN/25 mM HCOOH=85/15 (4)0.1 mL/min (5)40 °C (6)UV (254 nm) |
| VN-50 4D VN-50 2D |
(1)0.5 % Ethylene glycol (2)3 μL (VN-50 4D), 1 μL (VN-50 2D) (3)CH3CN/H2O=75/25 (4)0.6 mL/min (VN-50 4D), 0.1 mL/min (VN-50 2D) (5)30 °C (6)RI |
| NH2P-50 4B NH2P-50 4D NH2P-50 4E NH2P-50 2D |
(1)5 mg/mL Sucrose (2)3 μL (NH2P-50 4B), 6 μL (NH2P-50 4D, NH2P-50 4E), 1.5 μL (NH2P-50 2D) (3)CH3CN/H2O=75/25 (4)0.6 mL/min (NH2P-50 4B, NH2P-50 4D), 1.0 mL/min (NH2P-50 4E), 0.2 mL/min (NH2P-50 2D) (5)30 °C (NH2P-50 4B, NH2P-50 4D, NH2P-50 4E), 35 °C (NH2P-50 2D) (6)RI |
| NH2P-40 3E | (1)5 mg/mL Sucrose (2)4 μL (3)CH3CN/H2O=75/25 (4)0.35 mL/min (5)35 ℃ (6)RI |
| C18U 2B C18U 2D |
(1)0.24 mg/mL Naphthalene (2)1 μL (3)CH3CN/H2O=60/40 (4)0.4 mL/min (5)Ambient (6)UV (254 nm) |
| C18M 4D C18M 4E |
(1)0.61 mg/mL Naphthalene (2)1.5 μL (C18M 4D), 2 μL (C18M 4E) (3)CH3OH/H2O=70/30 (4)1.0 mL/min (5)30 °C (6)UV (254 nm) |
