Column Test Conditions
We are often asked “What standards should I run to assess the condition of my column?”. Well, here they are for all of our columns. We realize that chromatographers may have columns for years along with several changes of ownership and/or locations. Often, the original COA is lost. We can replace your COA on request. However, you can run the standard samples and asses the column quality even if you no longer have the original COA. Refer to the conditions below. Don’t hesitate to contact us if you need further assistance. Sometimes the column can be regenerated to produce “good as new” quality chromatograms.
| Column type | Test conditions (1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector |
|---|---|
| KW-802.5 to KW-804 | (1)0.2% Ethylene glycol (2)30μL (3)H2O (4)1.0mL/min (5)Ambient (6)RI |
| LW-803 | (1)BSA (2)1μL (3)50mM Phosphate buffer + 0.3M NaCl(pH7.0) (4)1.0mL/min (5)Ambient (6)UV(280nm) |
| KW402.5 to KW405 | (1)0.05% Uridine(KW402.5-4F, KW403-4F) 0.02% Uridine(2)1μL (3)50mM Phosphate buffer + 0.3M NaCl(pH7.0) (4)0.35mL/min (5)Ambient (6)UV(280nm) |
| SB-802 HQ to SB-806M HQ | (1)1% Ethylene glycol(802 HQ), 0.5% Ethylene glycol(802.5 HQ to 806M HQ) (2)10μL(802 HQ), 15μL(802.5 HQ to 806M HQ) (3)H2O (4)1.0mL/min (5)Ambient (6)RI |
| SB-807 HQ | (1)0.5% Glucose (2)10μL (3)H2O (4)1.0mL/min (5)Ambient (6)RI |
| GS-220 HQ GS-320 HQ GS-520 HQ GS-620 HQ | (1)1% Ethylene glycol (2)30μL (3)H2O (4)0.6mL/min (5)30°C (6)RI |
| GF-210 HQ GF-310 HQ GF-510 HQ GF-710 HQ GF-7M HQ | (1)0.2% Ethylene glycol(GF-210 HQ), 1% Ethylene glycol(Other) (2)20μL(GF-210 HQ), 30μL(Other) (3)H2O (4)0.6mL/min (5)Ambient(GF-210 HQ), 30°C(Other) (6)RI |
| GF-310 4B GF-310 4D GF-310 4E GF-310 2D | (1)0.5% Ethylene glycol (2)2μL(GF-310 4B, GF-310 4D), 3μL(GF-310 4E), 1μL(GF-310 2D) (3)H2O (4)0.25mL/min(GF-310 4B), 0.2mL/min(GF-310 4D, GF-310 4E), 0.05mL/min(GF-310 2D) (5)35°C (6)RI |
| KF-801 to KF-807L | (1)0.1% Propylbenzene (2)20μL (3)THF (4)1.0mL/min (5)Ambient (6)UV(254nm) |
| K-801 to K-807L (discontinued) | (1)0.1% Propylbenzene (2)20μL (3)Chloroform (4)1.0mL/min (5)Ambient (6)UV(254nm) |
| KD-801 to KD-807 | (1)1.0% Acetone (2)20μL (3)DMF (4)1.0mL/min (5)Ambient (6)RI |
| KF-601 to KF-607 (discontinued) | (1)0.5% Propylbenzene(KF-601), 0.1% Dicyclohexyl phthalate(KF-602 to KF-607) (2)5μL (3)THF (4)0.5mL/min (5)Ambient (6)UV(254nm) |
| HK-400 Series | (1)0.1% Phthalic acid dicyclohexyl (2)5μL (3)THF (4)0.3mL/min (5)40°C (6)UV(254nm) |
| KF-401HQ to KF-406LHQ | (1)0.5% Propylbenzene(KF-401HQ), 0.1% Dicyclohexyl phthalate(KF-402HQ to KF-406LHQ) (2)5μL (3)THF (4)0.3mL/min (5)Ambient (6)UV(254nm) |
| HFIP-803 to HFIP-807 (discontinued) | (1)5% Acetone (2)10μL (3)HFIP (4)1.0mL/min (5)Ambient (6)UV(280nm) |
| HFIP-603 to HFIP-607 (discontinued) | (1)5% Acetone (2)2μL (3)HFIP (4)0.25mL/min (5)Ambient (6)UV(280nm) |
| HT-803 to HT-807 (discontinued) | (1)10% Monochlorobenzene (2)20μL (3)Toluene (4)1.0mL/min (5)Ambient (6)RI |
| UT-802.5 UT-806M UT-807 (discontinued) | (1)10% Monochlorobenzene (2)10μL (3)Toluene (4)1.0mL/min (5)Ambient (6)RI |
| AT-806MS (discontinued) | (1)1% Acetone (2)20μL (3)Toluene (4)1.0mL/min (5)Ambient (6)RI |
| LF-804 LF-604 LF-404 | (1)0.1% Propylbenzene(LF-804), 0.1% Dicyclohexyl phthalate(LF-604, LF-404) (2)20μL(LF-804), 5μL(LF-604, LF-404) (3)THF (4)1.0mL/min(LF-804), 0.5mL/min(LF-604), 0.3mL/min(LF-404) (5)Ambient (6)UV(254nm) |
