Column Test Conditions for Ion Chromatography
We are often asked “What standards should I run to assess the condition of my column?”. Well, here they are for all of our columns. We realize that chromatographers may have columns for years along with several changes of ownership and/or locations. Often, the original COA is lost. We can replace your COA on request. However, you can run the standard samples and asses the column quality even if you no longer have the original COA. Refer to the conditions below. Don’t hesitate to contact us if you need further assistance. Sometimes the column can be regenerated to produce “good as new” quality chromatograms.
| Column type | Test conditions (1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector |
|---|---|
| YS-50 | (1)20mg/L Ca2+ (2)10μL (3)4mM Methanesulfonic acid aq. (4)1.0mL/min (5)40°C (6)CD |
| YK-421 | (1)20mg/L Ca2+ (2)15μL (3)5mM Tartaric acid + 1mM Dipicolinic acid + 24mM Boric acid aq. (4)1.0mL/min (5)40°C (6)CD |
| Y-521 (discontinued) | (1)20mg/L Na+ (2)50μL (3)4mM HNO3 aq. (4)1.5mL/min (5)40°C (6)CD |
| T-521 (discontinued) | (1)20mg/L Na+ (2)20μL (3)3mM HNO3 aq. (4)1.0mL/min (5)40°C (6)CD |
| NI-424 | (1)10mg/L SO42- (2)50μL (3)8mM 4-Hydroxybenzoic acid + 2.8mM Bis(2-hydroxyethyl)imino- tris(hydroxymethyl)methane + 2mM Phenylboronic acid + 5μM trans-1,2-Aminocyclohexane-N,N,N’,N’-4 acetic acid aq.(4)1.0mL/min (5)40°C (6)CD |
| I-524A | (1)10mg/L NO3- (2)30μL (3)2.5mM Phthalic acid aq.(pH4.0) (4)1.5mL/min (5)40°C (6)CD |
| SI-90 4E | (1)15mg/L SO42- (2)20μL (3)1.8mM Na2CO3 + 1.7mM NaHCO3 aq. (4)1.0mL/min (5)Ambient (6)Suppressed CD |
| SI-50 4E | (1)10mg/L Br- (2)20μL (3)3.2mM Na2CO3 + 1.0mM NaHCO3 aq. (4)0.7mL/min (5)Ambient (6)Suppressed CD |
| SI-52 4E | (1)40mg/L SO4- (2)50μL (3)3.6mM Na2CO3 aq. (4)0.8mL/min (5)45°C (6)Suppressed CD |
| SI-35 4D | (1)40mg/L SO4- (2)20μL (3)3.6mM Na2CO3 aq. (4)0.6mL/min (5)45°C (6)Suppressed CD |
