PROTEIN LW-403 4D is a rapid semi-micro column for aqueous SEC (GFC) suitable for antibody protein analysis. A comparison of IgG aggregate separation with a conventional device and a semi-micro type device was completed. The resolution of the conventional device is reduced due to the diffusion of the sample outside the column. We recommend using a semi-micro type device to achieve the best column performance.
Sample : 5 μL
IgG from human serum 2 mg/mL
|Semi-micro type||Conventional type|
|Tubing||0.1 mm I.D.||0.25 mm I.D.|
|UV detector||Cell volume||2.5 μL||18 μL|
|Optical path length||5 mm||10 mm|
Column : Shodex PROTEIN LW-403 4D (4.6 mm I.D. x 150 mm) Eluent : 50 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl Flow rate : 0.35 mL/min Detector : UV (280 nm) Column temp. : Room temp.