Compared with the case when Asahipak
NH2P
columns are used, when silica-based amino columns are used, detection sensitivity and plate number of saccharides are different according to the type of saccharides. And, the plate number of some specific saccharide such as galactose is extremely low.
Sample : 20micro-L
1. 2% Galactose
2. 1% Sucrose
3. 1% Maltose
Columns : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm),
Silica based amino column (other manufacturer)
Eluent : CH3CN/H2O=75/25
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 25°C
