Comparison of Sample Retention Using ODP, C8P and C4P Columns

The difference of retention of three columns for reversed phase chromatography, Asahipak ODP, C8P and C4P were compared.

Sample :
1. beta-Endorphin
2. Big gastrin
3. Insulin

 Columns : Shodex Asahipak ODP-50, C8P-50, C4P-50 (4.6mmID*150mm each)
 Eluent : 0.05% TFA aq./CH3CN=72/28
 Flow rate : 0.6mL/min
 Detector : UV(220nm)
 Column temp. : 30°C