The comparison of Asahipak GF-310 HQ ( a multisolvent GPC column ) and an ODS column when they were used for the measurement of POE distribution of octylphenol ethoxylate is shown here. When GF-310 HQ was used, good chromatograms were obtained using 35 and 40% acetonitrile concentration. On the other hand, when an ODS column was used, POE distribution can be measured using 55% acetonitrile concentration but the separation is not sufficient.
Sample :
Octylphenol ethoxylate
Column : Shodex Asahipak GF-310 HQ (7.6mmID*300mm)
Eluent : CH3CN/H2O
Flow rate : 0.6mL/min
Detector : Shodex UV(280nm)
Column temp. : 30°C
Column : ODS column from the other manufacturer
Eluent : CH3CN/H2O
Flow rate : 0.8mL/min
Detector : UV(280nm)
Column temp. : 30°C
