RSpak KC-811 6E is suitable for the analysis of cyanide ion and cyanogen chloride, fulfills required measurement for drinking-water regulation in Japan.
Post-column reagents preparation method
Reagent 1: 100mM phosphate buffer Containing 3.6 mM chloramine T.
Preparation of phosphate buffer:
1. Dissolve 3.40 g of Potassium dihydrogenphosphate in water to make up the volume 250 mL.
2. Dissolve 14.20 g of Disodium hydrogenphosphate in water to make up the volume 1L.
Mix 1 and 2.
Dissolve 0.5g Chloramin T in phosphate buffer to make up the volume 500 mL.
*Old Chloromin T may show rapid decomposition. In the case, keep the solution in the ice-bath.
Reagent 2: 0.03M 1-phenyl-3-methyl-5-pyrazolone+0.1M sodium 4-pyridinecarboxylate
1. Dissolve 2.5 g 1-Phenyl-3-mehyl-5-pyrazolone in 150mL N, N-dimethylformamide.
2. Dissolve 7.0 g sodium 4-pyridinecarboxylate tetrahydrate in 300 mL water.
Mix 1 & 2 and make up the volume 500 mL with water.
Store this solution under dark place, Temp. 
Sample : 10 µL each, 100 µL
CN–, Cyanide ion
CNCl, Cyanogen chloride
Column : Shodex RSpak KC-811 6E (6.0mmID*250mm)
Eluent : 1.0mM H2SO4 aq.
Reagent A : Chloramine T solution
Reagent B : 4-Pyridinecarboxylic acid-Pyrazolone solution
Flow rate : (Eluent); 1.0mL/min
(Reagent); 0.5mL/min each
Detector : VIS(638nm)
Column temp. : 40°C
Reaction temp. : (Reagent A); 40°C
(Reagent B); 80°C
