Japanese Notification Eishin No. 47* (May 23, 1996, Ministry of Health, Labour and Welfare (MHLW)) “Analytical Methods for Nutritional Components, Following the Introduction of Nutrition Labeling Standards” includes “Appendix: Analytical Methods for Nutritional Components”. Under section 8. Dietary Fiber, (2) High-Performance Liquid Chromatography Method (Enzyme-HPLC Method) targets foods containing low-molecular-weight water-soluble dietary fibers that are difficult to analyze using the Prosky method. In the enzyme-HPLC method, the indigestible fraction of trisaccharides and higher (indigestible oligosaccharides + dietary fiber fraction) is separated from other fractions (monosaccharides, disaccharides, and sugar alcohol fraction), and the ratio of the indigestible fraction is determined. Two columns, either ligand-exchange or gel-filtration types, are connected in series. Asahipak GS-220 HQ is listed as an example of a gel-filtration column. In this study, we used Asahipak GS-220 HQ to analyze low-molecular-weight water-soluble dietary fibers. For comparison, we also analyzed using a ligand-exchange column, SUGAR KS-802. GS-220 HQ enables easy separation of the indigestible fraction from other fractions in size-exclusion mode: in the chromatogram, the indigestible fraction elutes before the indicated arrow, while the other fractions elute after. On the other hand, KS-802 operates in both size-exclusion and ligand-exchange modes, which provides excellent separation among monosaccharides and disaccharides; however, its complex separation behavior makes it difficult to simply divide into indigestible and other fractions. Therefore, GS-220 HQ is highly effective for quantitative analysis of low-molecular-weight water-soluble dietary fibers.
- (Note)
- On April 1, 2015, the Food Labeling Act was enforced, and the Nutrition Labeling Standards were integrated into the Food Labeling Standards.
*The version at the time of the application acquisition.
Sample: 20 µL each
Low molecular weight water soluble dietary fiber
Columns : Shodex Asahipak GS-220 HQ (7.6mmID*300mm) x 2, Shodex SUGAR KS-802 (8.0mmID*300mm) x 2 Eluent : H2O, H2O Flow rate : 0.5mL/min, 0.3mL/min Detector : Shodex RI, Shodex RI Column temp. : 60°C, 80°C