Dipeptides

Dipeptides were analyzed using RSpak DE-613 (a column for reversed phase chromatography).

Sample :
1. Gly-Val
2. Phe-Gly
3. Ala-Phe
4. Val-Phe
5. Tyr-Leu

 Column : Shodex RSpak DE-613 (6.0mmID*150mm)
 Eluent : (A); 0.05% TFA aq./CH3CN=96/4
 (B); 0.05% TFA aq./CH3CN=75/25
 Linear gradient: 0 to 30min, (A) to (B)
 Flow rate : 1.0mL/min
 Detector : UV(220nm)
 Column temp. : 30°C