Several aldehydes were derivatized by DNPH (Dinitrophenylhydrazine) and analyzed using Asahipak ODP-50 4D.
Sample : 10μg/mL each, 20μL
1. Formaldehyde -2,4-DNPH
2. Acetaldehyde -2,4-DNPH
3. Acetone -2,4-DNPH
4. Propionaldehyde -2,4-DNPH
5. Isobutyraldehyde -2,4-DNPH
6. n-Butyraldehyde -2,4-DNPH
7. Isovaleraldehyde -2,4-DNPH
8. Valeraldehyde -2,4-DNPH
Column : Shodex Asahipak ODP-50 4D (4.6mmI.D. x 150mm) Eluent : CH3CN/H2O=45/55 Flow rate : 1.0mL/min Detector : UV(360nm) Column temp. : 40°C
