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Category: Monosaccharides and Oligosaccharides

Durability against Acidic Solvents


Since Asahipak NH2P-50 column is chemically stable,
the durability against acidic solvents is better than that of silica-based amino columns.

Testing procedure:

(1) Testing (63hrs)
 Eluent : 0.1M H2SO4 (pH2)
 Flow rate : 0.1mL/min
 Column temp. : Room temp.
 

(2) Replacement (1hr)
 Eluent : H2O
 Flow rate : 0.5mL/min
 Column temp. : Room temp.
 

(3) Equilibrium (1hr)
 Eluent : 0.1M NaOH
 Flow rate : 0.5mL/min
 Column temp. : Room temp.

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Comparison between before and after testing:

Sample Before testing After testing
Elution volume
(mL)		 Plate number Elution volume
(mL)		 Plate number
1.Fructose 6.73 9600 6.70 8400
2.Glucose 8.59 7900 8.58 8500
3.Sucrose 11.88 9600 11.93 9300
4.Maltose 14.24 8500 13.90 8600 



 Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
 Eluent : CH3CN/H2O=75/25
 Flow rate : 1.0mL/min
 Detector : Shodex RI
 Column temp. : 30°C

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