Durability against Alkaline Solvents

Since the packing material of Asahipak NH2P-50
column is polymer, it is durable against alkaline solvents. To the contrary,
silica-based amino columns are not durable against alkaline solvents.

Testing procedure:

(1)Equilibrium(1hr) Conditions before testing
 Eluent : 100mM Ammonium acetate(pH9.3)
 Flow rate : 0.1mL/min
 Column temp. : Room temp.

(2) Analysis

(3)Testing(160hrs)
 Eluent : 5mM NaOH(pH11.4)/CH₃CN=25/75
 Flow rate : 0.1mL/min
 Column temp. : 30°C

(4)Replacement(0.5hr)
 Eluent : H₂O
 Flow rate : 0.5mL/min
 Column temp. : Room temp.

(5)Replacement(0.5hr)
 Eluent : H₂O
 Flow rate : 0.5mL/min
 Column temp. : Room temp.

(6) Analysis

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Comparison between before and after testing:

Sample	Before testing		After testing
Sample	Elution volume (mL)	Plate number	Elution volume (mL)	Plate number
1.Fructose	7.52	7100	7.62	8100
2.Glucose	9.63	6100	9.68	7700
3.Sucrose	13.41	8400	13.40	9700
4.Maltose	16.16	7200	16.08	7400


 Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
 Eluent : CH₃CN/H₂O=75/25
 Flow rate : 1.0mL/min
 Detector : Shodex RI
 Column temp. : 30°C