Since the swelling and shrinking of the packing material of Asahipak
NH2P-50
columns is considerably small even when the eluent composition is
changed, any mixing ratio of acetonitrile and water can be used with
the NH2P-50 columns.
Comparison between before and after testing
| Sample | Before testing | After testing | ||
|---|---|---|---|---|
| Elution volume (mL) |
Plate number | Elution volume (mL) |
Plate number | |
| 1. Fructose | 7.61 | 11600 | 7.56 | 9800 |
| 2. Glucose | 9.80 | 7500 | 9.72 | 6200 |
| 3. Sucrose | 13.86 | 11700 | 13.75 | 9300 |
| 4. Maltose | 16.78 | 8300 | 16.66 | 6700 |
Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent : CH3CN/H2O=75/25
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 30°C
