The durability test for PROTEIN LW-403 4D, an aqueous SEC (GFC) semi-micro column for rapid analysis of antibody proteins, by the number of sample injections was conducted. It is confirmed that the column performance has barely changed after 500 injections.
Sample: 0.5μL
IgG from human serum 10mg/mL
Column : Shodex PROTEIN LW-403 4D (4.6mmI.D. x 150mm) Eluent : 50mM Sodium phosphate buffer (pH7.0) + 0.3M NaCl Flow rate : 0.35mL/min Detector : UV(280nm) (small cell volume) Column temp. : Room temp.
