The durability test for PROTEIN LW-403 4D, an aqueous SEC (GFC) semi-micro column for rapid analysis of antibody proteins, by the number of sample injections was conducted. It is confirmed that the column performance has barely changed after 500 injections.
IgG from human serum 10mg/mL
Column : Shodex PROTEIN LW-403 4D (4.6mmI.D. x 150mm) Eluent : 50mM Sodium phosphate buffer (pH7.0) + 0.3M NaCl Flow rate : 0.35mL/min Detector : UV(280nm) (small cell volume) Column temp. : Room temp.