Effect of Acetonitrile Concentration

Sugar alcohols were analyzed using SUGAR SC1211 (a column for saccharides analysis). When the concentration of acetonitrile in the eluent is increased, the retention of sample becomes stronger and separation is improved though the neccessary time for the analysis becomes longer.

Sample :
1. Maltitol
2. Glycerol
3. Mannitol
4. Xylitol
5. Sorbitol

 Column : Shodex SUGAR SC1211 (6.0mmID*250mm)
 Eluent : (A); H2O, (B); H2O/CH3CN=80/20, (C); H2O/CH3CN=60/40 
 Flow rate : 1.0mL/min
 Detector : Shodex RI
 Column temp. : 70°C