Effect of Column Length on Elution Pattern

Polystyrene standrads, n-propylbenzene and benzene were analyzed using GPC KF-801 (a column for GPC separation). Increasing the number of columns connected is an effective way of improving the resolution. In this case, the use of four columns improves the separation of polystyrene oligomer significantly.

Sample :
1. PS, Polystyrene (MW: 6,200)
2. PS, Polystyrene (MW: 300)
3. n-Propylbenzene
4. Benzene

 Columns : Shodex GPC KF-801 (8.0mmID*300mm)
 Eluent : THF
 Flow rate : 1mL/min
 Detector : UV(254nm)
 Column temp. : Room temp.