Effect of column temperature on elution volume when RSpak KC-811 is used is shown.
Sample :
1. Oxalic acid
2. Citric acid
3. Tartaric acid
4. Malonic acid
5. Succinic acid
6. Lactic acid
7. Formic acid
8. Acetic acid
9. Pyroglutamic acid
Column : Shodex RSpak KC-811 (8.0mmID*300mm)
Eluent : 0.1% H3PO4 aq.
Flow rate : 1.0mL/min
Column temp. : 28, 40, 60, 80°C
