Effect of Eluent Components

Instead of dichloromethane which may cause the environmental pollution, non-halogenated solvent such as ethyl acetate and acetone can be used as eluent. Each solvent is mixed with cyclohexane and used for CLNpak EV-2000 series as the eluent. The effect of the solvents and their mixing ratio is shown below.

Sample :
1. 125mg/mL Corn oil
2. 1mg/2mL beta-Carotene
3. 1mg/2mL Fluvalinate
4. 0.3mg/2mL Chinomethionate

 Columns : (Left); Shodex CLNpak EV-G AC (20mmID*100mm) + EV-2000 AC (20mmID*300mm) 
 (Center); Shodex CLNpak EV-2000 (20mmID*300mm)
 (Right); Shodex CLNpak EV-G (20mmID*100mm) + EV-2000 (20mmID*300mm)
 Eluent : (Left); Acetone/Cyclohexane
 (Center); Ethyl acetate/Cyclohexane
 (Right); Dichloromethane/Cyclohexane
 Flow rate : 4.0mL/min
 Detector : UV(254nm)
 Column temp. : 25°C