Effect of Eluent pH on Analysis Result

The RSpak DE series is stable in wide eluent pH range.

Sample : 10µL
1. Theobromine
2. Theophylline
3. Caffeine

 Column : Shodex RSpak DE-413 (4.6mmID*150mm) 
 Eluent : 10mM Sodium phosphate buffer/CH3CN=82/18
 Flow rate : 0.6mL/min 
 Detector : UV(254nm) 
 Column temp. : 40°C