Effect of Flow Rate (KW-802.5, KW-803)

Effects of flow rate when analyzing proteins using PROTEIN KW-802.5 and KW-803 (columns for aqueous SEC (GFC)) were shown.

Sample: BSA, Myoglobin, 0.2% each, 50μL

Columns      : (Left); Shodex PROTEIN KW-802.5, (Right); KW-803 (8.0mmI.D. x 300mm each)
Eluent       : 50mM Phosphate buffer + 150mM Na2SO4
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : 25°C