Effects of flow rate when analyzing proteins using PROTEIN KW-802.5 and KW-803 (columns for aqueous SEC (GFC)) were shown.
Sample: BSA, Myoglobin, 0.2% each, 50μL
Columns : (Left); Shodex PROTEIN KW-802.5, (Right); KW-803 (8.0mmI.D. x 300mm each) Eluent : 50mM Phosphate buffer + 150mM Na2SO4 Flow rate : 1.0mL/min Detector : UV(280nm) Column temp. : 25°C