Effect of Flow Rate on Elution Pattern

Polystyrene standard (Mw: 500) was analyzed using GPC KF-802 ( a column for GPC separation ). For improving resolution, the appropriate flow rate should be selected considering the diffusion speed. In this case, a higher resolution can be realized with a lower flow rate. Although a reduction of the flow rate from 2.0mL/min to 1.0mL/min brought about a large difference in resolution, a reduction from 1.0mL/min to 0.5mL/min did not. Considering the time for analysis, the use of 1.0mL/min is recommended for the analysis of the sample.

Sample : PS, Polystyrene 500

 Column : Shodex GPC KF-802 (8.0mmID*300mm) 
 Eluent : THF
 Flow rate : (A)2.0mL/min
 (B)1.0mL/min
 (C)0.5mL/min
 Detector : UV(254nm)
 Column temp. : Room temp.