Effects of flow rate when analyzing saccharides using SUGAR SC1011 (a column for saccharide analysis) are shown here.
It is confirmed that the theoretical plate number and resolution are improved by decreasing the flow rate.
Sample : 0.5%each, 5μL
1. Isomaltotriose
2. Sucrose
3. Glucose
4. Lactitol
5. Fructose
6. meso-Erythritol
7. Mannitol
8. Dulcitol
9. Xylitol .
Column : Shodex SUGAR SC1011 (8.0mmI.D. x 300mm) Eluent : H2O Detector : Shodex RI Column temp. : 80°C