Effect of Flow Rate (SC1211)

A mixture of oligosaccharides, sugar alcohols and ethanol is separated using SUGAR SC1211 (a column for saccharides analysis) with the flow rate of 1.0mL/min and 0.5mL/min. The separation can be improved using lower flow rate.

Sample : 5micro-L
1. 0.5% Pullulan P-10
2. 1.0% Maltotriose
3. 1.0% Maltose
4. 1.0% Glucose
5. 1.0% Fructose
6. 1.0% Glycerol
7. 2.0% Ethanol
8. 1.0% Sorbitol

 Columns : Shodex SUGAR SC1211 (6.0mmID*250mm)
 Eluent : H2O
 Flow rate : (A); 1.0mL/min, (B); 0.5mL/min
 Detector : Shodex RI
 Column temp. : 80°C