Effect of Organic Solvent in Eluent

Effect of organic solvent in eluent when saccharides are analyzed using Asahipak NH2P-50 4E (a column for saccharides analysis) is shown.

Sample :
1. Fructose
2. Glucose
3. Sucrose
4. Lactose
5. Maltose

 Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
 Eluent : (left); H2O/CH3CN=25/75
 (right); H2O/C2H5OH=10/90 
 Flow rate : 0.6mL/min
 Detector : Shodex RI
 Column temp. : 30°C