Effect of Organic Solvent in Eluent

Effect of organic solvent in eluent when saccharides are analyzed using Asahipak NH2P-50 4E (a column for saccharides analysis) is shown.

s0140
Sample :
1. Fructose
2. Glucose
3. Sucrose
4. Lactose
5. Maltose


 Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
 Eluent : (left); H₂O/CH₃CN=25/75
 (right); H₂O/C₂H₅OH=10/90 
 Flow rate : 0.6mL/min
 Detector : Shodex RI
 Column temp. : 30°C