Effects of pH on Retention Time (NI-424)
Effects of eluent pH on the elution of each anion using IC NI-424
(an anion analysis column for non-suppressor method) are shown. When the pH of the eluent is increased, the retention time for the anions is decreased.
Sample : 20?L
H2PO4– 10mg/L
F– 10mg/L
Cl– 10mg/L
NO2– 10mg/L
Br– 10mg/L
NO3– 10mg/L
I– 20mg/L
SO42- 10mg/L
S2O32- 50mg/L
SCN– 50mg/L
ClO4– 50mg/L
Column : Shodex IC NI-424 (4.6mmI.D. x 100mm)
Eluent : 8mM 4-Hydroxybenzoic acid + 2mM Phenylboronic acid
+ 0.005mM *CyDTA(pH adjusted with Bis-Tris)
Flow rate : 1.0mL/min
Detector : Non-suppressed conductivity
Column temp. : 40°C
*CyDTA: trans-1,2-Diaminocyclohexane-N,N,N',N'-tetraacetic acid