Effect of pH on Retention Time (NI-424)

Effects of pH on Retention Time (NI-424)

Effects of eluent pH on the elution of each anion using IC NI-424
(an anion analysis column for non-suppressor method) are shown. When the pH of the eluent is increased, the retention time for the anions is decreased.

Sample : 20?L
H₂PO₄^– 10mg/L
F^– 10mg/L
Cl^– 10mg/L
NO₂^– 10mg/L
Br^– 10mg/L
NO₃^– 10mg/L
I^– 20mg/L
SO₄^2- 10mg/L
S₂O₃^2- 50mg/L
SCN^– 50mg/L
ClO₄^– 50mg/L

 Column : Shodex IC NI-424 (4.6mmI.D. x 100mm)
 Eluent : 8mM 4-Hydroxybenzoic acid + 2mM Phenylboronic acid
 + 0.005mM *CyDTA(pH adjusted with Bis-Tris)
 Flow rate : 1.0mL/min
 Detector : Non-suppressed conductivity
 Column temp. : 40°C
 
 *CyDTA: trans-1,2-Diaminocyclohexane-N,N,N',N'-tetraacetic acid