Anion standards were analyzed using IC I-524A. This shows the change in column efficiency with the concentration of the sample when the injection volume is 100micro-L. The column efficiency does not decrease until the sample concentration reaches 10mg/L for Cl
– and 100mg/L for SO
42-. Beyond those concentrations, HETP increases and peak becomes broad. When 1mg/L of Cl
– is injected, there is not decreasing in column efficiency until the injection volume reaches 500micro-L.

Sample :
Cl–
SO42-
HETP=(Column length)/(Theoretical plate number)
Column : Shodex IC I-524A (4.6mmID*100mm)
Eluent : 2.5mM Phthalic acid(pH4.0 adjusted by Tris(hydroxymethyl)aminomethane)
Flow rate : 1.2mL/min
Detector : Shodex CD
Column temp. : 40°C