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Effect of Sample Solvent Composition

Effect of sample solvent composition when Asahipak
NH2P-50 is used is shown.
When making sample solution to be injected, sample should be
dissolved in a solvent whose composition is as close as the eluent,
preferably in the eluent. When 75% acetonitrile solvent is used for
the eluent and a sample cannot be dissolved in it, first, the sample
should be dissolved in a solvent which dissolves the sample, and then,
adjust the solvent composition to be acetonitrile aqueous solution of 50% or more.

Sample : 1mg/mL each, 20micro-L
1. meso-Erythritol 2. Xylitol 3. Fructose 4. Glucose 5. Sucrose 6. Lactose 7. Maltose

Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent : CH3CN/H2O=75/25
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 25°C