The effects of flow rate for protein analysis with IEC SP-FT 4A, a column for cation exchange chromatography, were investigated. The particle size of SP-FT 4A is small (2.7μm) but the pressure of the column can be suppressed by advanced control for particle size. Since the pressure is under 20MPa at 3.0mL/min flow rate, SP-FT 4A can provide ultra-rapid and high-resolution analysis using conventional devices as same as when using UHPLC.
(Caution) Backpressure will differ depends on the LC system used and the analytical conditions such as eluent compositions and column temperature. Please set the system pressure (sum of column and system pressures) less than 20 MPa.
Sample : 5μL
1. α-Chymotrypsinogen A
2. Ribonuclease A
Column : Shodex IEC SP-FT 4A (4.6mm I.D. x 10mm) Eluent : (A); 20mM *MES buffer (pH5.6) (B); (A) + 0.5M Na2SO4 Linear gradient: (A) to (B) (2min) Flow rate : 1.0, 1.5, 3.0mL/min Detector : UV(280nm) Column temp. : Room temp. *MES : 2-(N-Morpholino) ethanesulfonic acid