Polystyrene standards (Mp580) were analyzed using GPC HK-401, an organic SEC (GPC) column for ultra-rapid analysis. Comparing the separation between an HK-401 with a conventional SEC column of equivalent at the flow rate of 1.0 mL/min, HK-401 achieved the same level of separation with 1/6 analysis time. However, when higher separation is desired, use of a lower flow rate is recommended.
Sample : 0.5 %, 5 µL
1. Polystyrene (Mp: 580)
Columns : Shodex GPC HK-401 (4.6 mm I.D. x 150 mm) x 2 Eluent : THF Flow rate : 1.0 mL/min, 0.6 mL/min, 0.3 mL/min Detector : RI (small cell volume) Column temp. : 30 °C