Effects of Flow Rate on Separation (HK-406: PS)

Polystyrene standards were analyzed using GPC HK-406, an organic SEC (GPC) column for ultra-rapid analysis. Comparing the separation between an HK-406 with a conventional SEC column of equivalent at the flow rate of 1.0 mL/min, HK-406 achieved the same level of separation with 1/6 analysis time. However, when higher separation is desired, the use of a lower flow rate is recommended.

Sample : 0.5 %, 5 µL

1. Polystyrene (Mp: 609,000)

2. Polystyrene (Mp: 53,000)

3. Polystyrene (Mp: 3,180)

s2039

Columns      : Shodex GPC HK-406 (4.6 mm I.D. x 150 mm) x 2
Eluent       : THF
Flow rate    : 1.0 mL/min, 0.6 mL/min, 0.3 mL/min
Detector     : RI (small cell volume)
Column temp. : 30 °C