Main separation mode for the GPC clean-up is size exclusion mode: based on
analytes’ size differences, analytes are eluted in the order of their sizes.
However, by optimizing the mobile phase composition, partition/adsorption mode
can be also used. Change in acetone and cyclohexane ratios does not influence
elution volume of lipid much. However by decreasing acetone, retention time of
pesticides increases. At acetone/cyclohexane=3/7, lipid and acrinathirin co-elutes.
Sample : 40mg/L each, 5mL
Column : Shodex CLNpak EV-G AC (20.0mmID*100mm) + EV-2000 AC (20.0mmID*300mm)
Eluent : Acetone/Cyclohexane
Flow rate : 5mL/min
Detector : UV(254nm)
Column temp. : 40°C
