The effect of column temperature on HETP was examined for organic solvent SEC (GPC) semi-micro column,
KF-402HQThere is not a considerable temperature influence for the analysis of relatively small MW compound such as dicyclohexylphthalate. However, improved separation can be expected for the analysis of larger MW compound analysis at higher column temperature conditions.
Sample : 0.05% Dicyclohexylphthalate 5µ L
HETP= (Column length)/(Theoretical plate number)
Column : Shodex GPC KF-402HQ (4.6mmID*250mm) x 2
Eluent : THF
Flow rate : 0.3mL/min
Detector : UV(254nm)
