Two different temperature settings (ambient and 40 °C) were compared for the
GPC clean-up method using CLNpak
EV-2000 AC .
At ambient temperature, the high viscosity causes peak broadening and increases
the analysis time. Therefore, analysis at 40°C is preferred.
Sample : 5mL
Column : Shodex CLNpak EV-G AC (20.0mmID*100mm) + EV-2000 AC (20.0mmID*300mm)
Eluent : Acetone/Cyclohexane=1/4
Flow rate : 5mL/min
Detector : UV(254nm)