Effects of Temperature on Retention Time (SI-36 4D)

Please use this column with a suppressor-type ion chromatography system.

The relationship between column temperature and retention time of each anion in the anion analysis column IC SI-36 4D is shown below. When the temperature is increased, the elution time of sulfate ions decreased. At 40 °C or higher, the elution orders of sulfate ion and bromide ion are reversed. Considering the elution time of carbonate, we recommend a column temperature of 30 °C and the use of 25 mM potassium hydroxide aqueous solution as eluent. The relationship between column temperature and retention time of each anion in the anion analysis column SI-36 4D is shown below. The elution behavior with temperature depends on the kind of anion. The higher the temperature, the slower the elution of sulfate ions. At 40 °C or higher, the elution orders of sulfate ion and bromide ion are reversed. Considering the elution time of carbonate, a column temperature of 30 °C is recommended, when using 25 mM potassium hydroxide aqueous solution as eluent.

Sample: 25 μL

1. F^– 0.5 mg/L
2. Cl^– 3 mg/L
3. NO₂^– 5 mg/L
4. SO₄^2- 10 mg/L
5. Br^– 10 mg/L
6. NO₃^– 10 mg/L
7. PO₄^3- 20 mg/L

Column       : Shodex IC SI-36 4D (4.0 mm I.D. x 150 mm)
Eluent       : 25 mM KOH aq.
               (Eluent source : DionexTM EGC 500 KOH)
Flow rate    : 0.7 mL/min
Detector     : Suppressed conductivity
Column temp. : 30. 35, 40, 45, 50 °C