Elution volume for lipid and lipid acids were monitored by CLNpak
EV-2000 AC .
Lipid and lipid acids elute fast, thus by fractionating between acrinathrin and
tricyclazol, most lipid and lipid acids can be removed.
Sample : 5mL
Beef tallow
Acrinathrin
Tricyclazole
Oleic acid
Palmitic acid
Linolenic acid
Linoleic acid
Column : Shodex CLNpak EV-G AC (20.0mmID*100mm) + EV-2000 AC (20.0mmID*300mm)
Eluent : Acetone/Cyclohexane=1/4
Flow rate : 5mL/min
Detector : UV(254nm)
Column temp. : 40°C
