Elution volume for lipid and lipid acids (EV-2000 AC)

Elution volume for lipid and lipid acids were monitored by CLNpak
EV-2000 AC .
Lipid and lipid acids elute fast, thus by fractionating between acrinathrin and
tricyclazol, most lipid and lipid acids can be removed.

Sample : 5mL

Beef tallow
Acrinathrin
Tricyclazole
Oleic acid
Palmitic acid
Linolenic acid
Linoleic acid

 
 Column : Shodex CLNpak EV-G AC (20.0mmID*100mm) + EV-2000 AC (20.0mmID*300mm)
 Eluent : Acetone/Cyclohexane=1/4
 Flow rate : 5mL/min
 Detector : UV(254nm)
 Column temp. : 40°C