Elution Volume of Nucleic Acid

The elution volume of Nucleic acids in taste components analyzed with Asahipak GS-320 7G (a multimode column) and an ODS column were compared .
GS-320 7G is an old type of GS-320 HQ.

Sample :
Adenine, Adenosine, AMP, CMP, Cytidine, Cytosine, GMP, Guanine, Guanosine, Hypoxanthine
IMP, Inosine, Thymidine, Thymine, TMP, UMP, Uracil, Uridine

 Columns : Shodex Asahipak GS-320 7G (7.6mmID*500mm), ODS column (6.0mmID*150mm)
 Eluent : 10mM Sodium phosphate buffer(pH2.5 to 10)
 Flow rate : 1.0mL/min (GS-320 7G), 0.6mL/min (ODS column)
 Detector : UV(260nm)
 Column temp. : 30°C