Elution volume of organic acids using RSpak NN-814 is shown.
| Substance | Elution Volume (mL) |
|---|---|
| Oxalic acid |
7.6
|
| Maleic acid | 8.8 |
| D-Gluconic acid | 8.9 |
| Gluconic acid | 9.0 |
| alpha-Ketoglutaric acid | 9.2 |
| Tartaric acid | 9.2 |
| Pyruvic acid | 9.4 |
| Citric acid | 9.5 |
| Glyoxylic acid | 9.7 |
| Malic acid | 9.8 |
| Glycolic acid | 9.8 |
| Malonic acid | 10.0 |
| Pyroglutamic acid | 10.0 |
| Substance | Elution Volume (mL) |
|---|---|
| Lactic acid | 10.2 |
| Formic acid | 10.2 |
| Citraconic acid | 10.7 |
| Succinic acid | 10.8 |
| Acetic acid | 10.8 |
| Levulinic acid | 11.1 |
| Glutaric acid | 11.7 |
| Itaconic acid | 12.3 |
| Adipic acid | 12.9 |
| Fumaric acid | 13.4 |
| trans-Crotonic acid | 15.2 |
| n-Capronic acid | 30.8 |
| L-Glutamic acid | 37.8 |
Column : Shodex RSpak NN-814 (8.0mmID*250mm)
Eluent : 0.1% H3PO4 aq.
Column temp. : 60°C
