Endorphins were analyzed using Asahipak GS-320 HQ ( a multimode column ).
Sample :
1. beta-Endorphin
2. alpha-Endorphin
3. gamma-Endorphin
Column : Shodex Asahipak GS-320 HQ (7.6mmID*300mm)
Eluent : 0.1% TFA/CH3CN=95/5
Flow rate : 0.8mL/min
Detector : UV(210nm)
Column temp. : 30°C