Fatty acid methyl esters were analyzed using RSpak DS-413, a polymer-based reversed-phase column.
Sample : 0.2% each, 20μL
1. Methyl linoleate
2. Methyl palmitate
3. Methyl oleate
4. Methyl stearate
Column : Shodex RSpak DS-413 (4.6mmI.D. x 150mm) Eluent : CH3CN/THF/H2O=45/30/25 Flow rate : 1.0mL/min Detetor : Shodex RI Column temp. : 40°C