Fatty Acids (4) (RP18-413)

Fatty acids were analyzed using RSpak RP18-413 (a column for reversed phase chromatography). Capric acid could be eluted after V₀ with addition of 0.1% H₃PO₄ to eluent.

Sample : C_nH_2n+1COOH, 30micro-L
1. Capric acid (n=9)
2. Lauric acid (n=11)
3. Myristic acid (n=13)
4. Palmitic acid (n=15)
5. Stearic acid (n=17)
6. Arachidic acid, Eicosanoic acid (n=19)

 Column : Shodex RSpak RP18-413 (4.6mmID*150mm)
 Eluent : 0.1% H3PO4 aq./CH3CN/THF=30/50/20
 Flow rate : 0.7mL/min
 Detector : Shodex RI
 Column temp. : 40°C